');mask-image:url('data:image/svg+xml;utf8, ');content:'';margin:0 .4rem;display:inline-block;vertical-align:middle;line-height:1}.breadcrumb-trail .trail-items li:last-child:after{display:none}.bloghash-breadcrumbs{font-size:1.4rem;border-width:0}#page .page-header .bloghash-breadcrumbs a>span{color:inherit}#bloghash-copyright .bloghash-flex-row>div:empty{display:none}#bloghash-copyright>.bloghash-container>.bloghash-flex-row>div{width:auto;padding-top:.6rem;padding-bottom:.6rem}.bloghash-copyright-layout-1 #bloghash-copyright>.bloghash-container>.bloghash-flex-row>div{-ms-flex-preferred-size:100%;flex-basis:100%;-ms-flex-negative:0;flex-shrink:0;-webkit-box-pack:center;-ms-flex-pack:center;justify-content:center}#bloghash-scroll-top{-webkit-box-align:center;-ms-flex-align:center;align-items:center;-webkit-box-pack:center;-ms-flex-pack:center;justify-content:center;position:fixed;bottom:2.5rem;right:2rem;z-index:997;width:4rem;height:4rem;opacity:0;-webkit-transform:translate3d(0,3rem,0);transform:translate3d(0,3rem,0)}#bloghash-scroll-top .bloghash-scroll-icon{overflow:hidden;z-index:2}#bloghash-scroll-top .bloghash-icon{-webkit-box-align:center;-ms-flex-align:center;align-items:center;-webkit-box-pack:center;-ms-flex-pack:center;justify-content:center;height:2rem;color:var(--bloghash-white);-webkit-transform:translateY(1.1rem);-ms-transform:translateY(1.1rem);transform:translateY(1.1rem)}#bloghash-scroll-top:before{content:'';z-index:1;border-radius:10rem;background-color:var(--bloghash-primary);-webkit-transform:scale(1);-ms-transform:scale(1);transform:scale(1);border:.1rem solid rgba(185,185,185,.4)}#bloghash-scroll-top svg{fill:var(--bloghash-white)}.bloghash-widget.widget:not(.widget_text):not(.hester-core-custom-list-widget) ol{list-style:none;margin-left:0;margin-right:0}#main .bloghash-widget{overflow:hidden}#main .bloghash-widget{margin-bottom:4rem}.widget ol{line-height:1.5}.widget ol:last-child{margin-bottom:0}.widget ol li{margin-bottom:1rem}.widget.bloghash-entry p{margin-top:1.6rem;margin-bottom:1.6rem}.widget.bloghash-entry p:last-child{margin-bottom:0}.widget.widget_block li a{position:relative;z-index:0}.widget.widget_recent_comments li{margin-bottom:1.6rem}.bloghash-entry>:first-child{margin-top:0}.bloghash-entry>:last-child{margin-bottom:0}.bloghash-entry ol{margin-left:4rem}.bloghash-entry ol{margin-bottom:2rem;margin-top:2rem}.bloghash-entry p{margin-bottom:1.5rem;margin-top:1.5rem}.author-avatar img{border-radius:var(--bloghash-full-radius);width:3rem;vertical-align:middle;margin-right:.8rem;display:inline-block;outline:1px solid rgba(185,185,185,.4)}#main .content-area .entry-meta a{color:inherit}.entry-meta{font-size:1.3rem;font-weight:600;margin-top:1.2rem}.entry-meta .bloghash-icon{height:1.827rem;margin-right:.7rem;vertical-align:text-bottom}.entry-meta .entry-meta-elements>span{padding:0 1rem;line-height:1.2}.entry-meta .entry-meta-elements>span.posted-on{line-height:1.5}.entry-meta .entry-meta-elements>span,.entry-meta .entry-meta-elements>span .posted-by{-js-display:inline-flex;display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex;-webkit-box-align:center;-ms-flex-align:center;align-items:center}.entry-meta .entry-meta-elements>span:first-child{padding-left:0}.entry-meta .entry-meta-elements>span:last-child{margin-right:0;padding-right:0}.entry-meta .entry-meta-elements>span:before{content:'';position:relative;left:-1.1rem;width:4px;height:4px;border-radius:100%;background-color:var(--bloghash-primary)}.entry-meta .entry-meta-elements>span:first-child:before{display:none}.entry-media{margin-bottom:2.5rem}.entry-media img{margin:0 auto;display:block}.single-post .entry-content{margin-top:4rem}.single-post .entry-media{margin-bottom:4rem}.single .post-category{margin-bottom:.3rem;font-size:1.4rem}.single .post-category a{-js-display:inline-flex;display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex;margin:0 .4rem .8rem}.single .entry-header{text-align:start;margin-bottom:1rem}.single .entry-meta{text-align:start;margin-top:1.6rem}.single .entry-content{margin-bottom:5rem}.single .post-nav .nav-previous .nav-content span{padding-right:2rem}.single .post-nav .nav-next .nav-content span:not(.ss-on-media-image-wrap){text-align:end;padding-left:2rem}.single.bloghash-page-title-align-left .bloghash-article>.entry-header,.single.bloghash-page-title-align-left .bloghash-article>.post-category{text-align:start}.single.bloghash-page-title-align-left .bloghash-article>.entry-meta>.entry-meta-elements{-webkit-box-pack:start;-ms-flex-pack:start;justify-content:flex-start}#cancel-comment-reply-link{font-size:1.3rem;border-radius:var(--bloghash-normal-radius);border:.2rem solid currentColor;font-weight:500;padding:.4rem .7rem;line-height:1;text-decoration:none;text-transform:capitalize;margin-left:1.4rem;color:inherit}button.bloghash-animate-arrow{-webkit-appearance:none;border:none;-webkit-box-shadow:none;box-shadow:none;background:0 0;outline:none}.bloghash-animate-arrow .arrow-bar{-webkit-transform:scaleX(0);-ms-transform:scaleX(0);transform:scaleX(0)}.bloghash-animate-arrow svg{vertical-align:middle}.bloghash-animate-arrow.right-arrow svg{-webkit-transform:rotate(180deg);-ms-transform:rotate(180deg);transform:rotate(180deg)}.bloghash-animate-arrow.right-arrow .arrow-bar{-webkit-transform-origin:100% 0;-ms-transform-origin:100% 0;transform-origin:100% 0}.bloghash-animate-arrow.right-arrow .arrow-handle{-webkit-transform:translateX(.6rem);-ms-transform:translateX(.6rem);transform:translateX(.6rem)}.bloghash-hamburger{padding:0;display:inline-block;font:inherit;color:inherit;text-transform:none;background-color:transparent;border:0;margin:0;overflow:visible}.hamburger-box{width:2.5rem;height:1.4rem;display:inline-block;position:relative}.hamburger-inner{display:block;top:50%;margin-top:-.1rem}.hamburger-inner,.hamburger-inner:before,.hamburger-inner:after{width:2.8rem;height:2px;background-color:#111827;border-radius:var(--bloghash-normal-radius);position:absolute}.hamburger-inner:before,.hamburger-inner:after{content:"";display:block}.hamburger-inner:before{top:-5px}.hamburger-inner:after{bottom:-5px}.bloghash-mobile-nav{order:1;display:none;margin-left:2.4rem}.bloghash-hamburger,.bloghash-mobile-nav{-webkit-box-align:center;-ms-flex-align:center;align-items:center}body:not(.is-mobile-menu-active) .bloghash-hamburger .hamburger-box .hamburger-inner:before{width:1.5rem}.bloghash-hamburger{-js-display:inline-flex;display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex;border-radius:var(--bloghash-normal-radius)}.bloghash-hamburger .hamburger-inner,.bloghash-hamburger .hamburger-inner:before,.bloghash-hamburger .hamburger-inner:after{background-color:currentColor}#bloghash-header:after{content:'';position:fixed;top:100%;left:0;right:0;height:100vh;background-color:rgba(255,255,255,.85);z-index:991;opacity:0;visibility:hidden;will-change:opacity,visibility;-webkit-transform:translate3d(0,0,0);transform:translate3d(0,0,0)}@media only screen and (min-width:600px){.site .bloghash-flex-row .start-sm{-webkit-box-pack:start;-ms-flex-pack:start;justify-content:flex-start;text-align:start}.site .bloghash-flex-row .end-sm{-webkit-box-pack:end;-ms-flex-pack:end;justify-content:flex-end;text-align:end;margin-left:auto}}@media only screen and (min-width:782px){.site .bloghash-flex-row .col-md{-webkit-box-flex:1;-ms-flex-positive:1;flex-grow:1;-ms-flex-negative:1;flex-shrink:1;max-width:100%;-ms-flex-preferred-size:0;flex-basis:0}.site .bloghash-flex-row .col-md.flex-basis-auto{-ms-flex-preferred-size:auto;flex-basis:auto}.site .bloghash-flex-row .end-md{-webkit-box-pack:end;-ms-flex-pack:end;justify-content:flex-end;text-align:end;margin-left:auto}}@media only screen and (max-width:868px){.bloghash-hide-mobile-tablet{display:none!important}.bloghash-container{padding:0 3rem}.bloghash-header-widgets .bloghash-header-widget,.bloghash-header-widgets .bloghash-header-widget.bloghash-header-widget__button{padding-left:1rem;padding-right:1rem}.bloghash-mobile-nav{margin-left:1.6rem}}@media only screen and (max-width:599px){#bloghash-header-inner .bloghash-widget-wrapper,.bloghash-header-element,.bloghash-header-widgets .bloghash-header-widget{position:static}.bloghash-header-widgets .dropdown-item:after{display:none}.page-header>.bloghash-container{-webkit-box-align:start;-ms-flex-align:start;align-items:flex-start;-webkit-box-orient:vertical;-webkit-box-direction:normal;-ms-flex-direction:column;flex-direction:column}.author-avatar img{display:none}}@media only screen and (max-width:480px){#page{min-height:-webkit-fill-available}}@media only screen and (max-width:960px){#bloghash-topbar>.bloghash-container>.bloghash-flex-row>div .bloghash-topbar-widget{padding-top:.6rem;padding-bottom:.6rem}#bloghash-topbar .bloghash-topbar-widget{padding-left:.8rem;padding-right:.8rem}.bloghash-topbar__separators-regular #bloghash-topbar .bloghash-topbar-widget:after{margin-left:.8rem}#main>.bloghash-container{display:block}#primary{max-width:100%!important;padding-left:0!important;padding-right:0!important;margin-top:4rem}.site-main #secondary{width:100%;margin-top:4rem}}@media all and (-ms-high-contrast:none),(-ms-high-contrast:active){.site .bloghash-flex-row:after{content:'';display:block;min-height:inherit;font-size:0}.bloghash-btn{height:1rem}#main>.bloghash-container #primary{-ms-flex-preferred-size:0%;flex-basis:0%}}.bloghash-btn{background-color:var(--bloghash-primary)}#bloghash-topbar .bloghash-topbar-widget__text .bloghash-icon{color:var(--bloghash-primary)}#masthead .bloghash-header-widgets .dropdown-item:after,.bloghash-nav>ul .sub-menu:after{border-bottom-color:var(--bloghash-primary);outline:none!important}.bloghash-header-widgets .dropdown-item,.bloghash-nav .sub-menu{border-top-color:var(--bloghash-primary)}::-webkit-selection{background-color:var(--bloghash-primary);color:var(--bloghash-white)}::-webkit-scrollbar-thumb{background:rgba(0,0,0,.2)}::-webkit-scrollbar-thumb:hover{background:rgba(0,0,0,.4)}.mr-1{margin-right:.4rem}a.bloghash-btn i{font-size:105%;vertical-align:baseline}a.bloghash-btn i:before{display:inline-block;vertical-align:middle}a.bloghash-btn span{vertical-align:baseline;line-height:normal}@media (prefers-reduced-motion:reduce){*{animation-duration:0s!important}}.bloghash-glassmorphism{position:fixed;right:0;left:0;bottom:0;display:flex;overflow:hidden;z-index:-1}.bloghash-glassmorphism .block{width:28.8rem;height:28.8rem;filter:blur(64px);mix-blend-mode:multiply;border-radius:999px;display:block;opacity:.1}.bloghash-glassmorphism .block.one{margin-top:5rem;background-color:rgba(239,35,60)}.bloghash-glassmorphism .block.two{margin-top:16rem;margin-left:-8rem;background-color:rgba(4,134,139)}@media only screen and (min-width:768px){.bloghash-glassmorphism{top:4rem}}@media only screen and (min-width:1024px){.bloghash-glassmorphism .block{width:38.4rem;height:38.4rem}}@media only screen and (min-width:1280px){.bloghash-glassmorphism{top:8rem}}.post-category .cat-links a.cat-31{color:#f43676;background:#fdd7e4}:root{--bloghash-primary:#f43676;--bloghash-primary_80:#fdd7e4;--bloghash-primary_15:#f6558b;--bloghash-primary_27:rgba(244,54,118,.27);--bloghash-primary_10:rgba(244,54,118,.1)}#bloghash-topbar{background:#f43676;background:-webkit-linear-gradient(45deg,#f43676 0,rgba(226,181,181,.39) 100%);background:-o-linear-gradient(45deg,#f43676 0,rgba(226,181,181,.39) 100%);background:linear-gradient(45deg,#f43676 0,rgba(226,181,181,.39) 100%)}#bloghash-topbar{border-style:solid}.bloghash-topbar-widget:after{background-color:#ccc}#bloghash-topbar{color:#002050}.bloghash-topbar-widget .bloghash-nav>ul>li>a,#bloghash-topbar .bloghash-topbar-widget__text .bloghash-icon{color:#302d55}#bloghash-header-inner{background:#fff}#bloghash-header,.bloghash-header-widgets a:not(.bloghash-btn),.bloghash-logo a,.bloghash-hamburger{color:#131315}#bloghash-header-inner{border-color:rgba(185,185,185,.4);border-bottom-width:1px}.bloghash-header-widget:after{background-color:#ccc}@media screen and (max-width:960px){#bloghash-header-inner .bloghash-nav{display:none;color:#000}.bloghash-mobile-toggen,.bloghash-mobile-nav{display:inline-flex}#bloghash-header-inner{position:relative}#bloghash-header-inner .bloghash-nav>ul>li>a{color:inherit}#bloghash-header-inner .site-navigation{display:none;position:absolute;top:100%;width:100%;height:100%;min-height:100vh;left:0;right:0;margin:-1px 0 0;background:#fff;border-top:1px solid #eaeaea;box-shadow:0 15px 25px -10px rgba(50,52,54,.125);z-index:999;font-size:1.7rem;padding:0}#bloghash-header-inner .site-navigation>ul{overflow-y:auto;max-height:68vh;display:block}#bloghash-header-inner .site-navigation>ul>li>a{padding:0!important}#bloghash-header-inner .site-navigation>ul li{display:block;width:100%;padding:0;margin:0;margin-left:0!important}#bloghash-header-inner .site-navigation>ul .sub-menu{position:static;display:none;border:none;box-shadow:none;border:0;opacity:1;visibility:visible;font-size:1.7rem;transform:none;background:#f8f8f8;min-width:initial;left:0;padding:0;margin:0;border-radius:0;line-height:inherit}#bloghash-header-inner .site-navigation>ul .sub-menu>li>a>span{padding-left:50px!important}#bloghash-header-inner .site-navigation>ul .sub-menu a>span{padding:10px 30px 10px 50px}#bloghash-header-inner .site-navigation>ul a{padding:0;position:relative;background:0 0}#bloghash-header-inner .site-navigation>ul li{border-bottom:1px solid #eaeaea}#bloghash-header-inner .site-navigation>ul>li:last-child{border-bottom:0}#bloghash-header-inner .site-navigation>ul a>span{padding:10px 30px!important;width:100%;display:block}#bloghash-header-inner .site-navigation>ul a>span:after,#bloghash-header-inner .site-navigation>ul a>span:before{display:none!important}#bloghash-header-inner .site-navigation>ul .menu-item-has-children>a{display:inline-flex;width:100%;max-width:calc(100% - 50px)}#bloghash-header-inner .bloghash-nav .menu-item-has-children>a>span{border-right:1px solid rgba(185,185,185,.4)}#bloghash-header-inner .bloghash-nav .menu-item-has-children>a>.bloghash-icon{transform:none;width:50px;margin:0;position:absolute;right:0;height:1em;display:none}.bloghash-mobile-toggen{width:50px;height:1em;background:0 0;border:none}.bloghash-mobile-toggen .bloghash-icon{transform:none;width:50px;margin:0;position:absolute;right:0;height:1em}}.bloghash-nav.bloghash-header-element,.bloghash-header-layout-1 .bloghash-header-widgets{font-weight:600;font-family:"Inter Tight",Helvetica,Arial,sans-serif;font-size:1.7rem;line-height:1.5}#secondary{width:30%}body:not(.bloghash-no-sidebar) #primary{max-width:70%}.bloghash-layout__boxed-separated #content>article,.bloghash-layout__boxed-separated.bloghash-sidebar-style-2 #secondary .bloghash-widget{background-color:#fff}body{color:#002050}h1,a,.entry-meta{color:#302d55}:root{--bloghash-secondary:#302d55}.bloghash-container{max-width:1480px}.bloghash-logo img{max-height:45px}.bloghash-logo .logo-inner{margin-top:27px;margin-right:10px;margin-bottom:27px;margin-left:10px}@media only screen and (max-width:768px){.bloghash-logo .logo-inner{margin-top:25px;margin-right:1px;margin-bottom:25px}}.page-header{background:rgba(244,54,118,.1)}.page-header{border-color:rgba(0,0,0,.062);border-style:solid;border-bottom-width:1px}.bloghash-breadcrumbs{padding-top:15px;padding-bottom:15px}html{font-size:62.5%}@media only screen and (max-width:768px){html{font-size:53%}}@media only screen and (max-width:480px){html{font-size:50%}}*{-moz-osx-font-smoothing:grayscale;-webkit-font-smoothing:antialiased}body{font-weight:400;font-family:"Be Vietnam Pro",Helvetica,Arial,sans-serif;font-size:1.7rem;line-height:1.75}h1{font-weight:700;font-style:normal;text-transform:none;text-decoration:none;font-family:"Be Vietnam Pro",Helvetica,Arial,sans-serif}h1{font-weight:700;font-size:4rem;line-height:1.4}.single-post .entry-content{font-size:1.6rem}.bloghash-btn{color:#fff;border-color:rgba(0,0,0,.12);border-width:.1rem;border-top-left-radius:.8rem;border-top-right-radius:.8rem;border-bottom-right-radius:.8rem;border-bottom-left-radius:.8rem}.bloghash-btn{font-weight:500;font-family:"Be Vietnam Pro",Helvetica,Arial,sans-serif;font-size:1.8rem}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-ss-on-media-button,.ss-popup-overlay .ss-copy-action .ss-button,.ss-popup-overlay .ss-popup .ss-close-modal,.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a{border:0;text-shadow:none;-webkit-box-shadow:none;box-shadow:none;outline:0;text-decoration:none;text-transform:none;letter-spacing:0}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-pinit-button,.ss-social-icons-container>li .ss-ss-on-media-button:after{outline:0}.ss-svg-icon{height:16px;width:auto;vertical-align:text-bottom;fill:currentColor}.ss-envelope-color{background-color:#323b43}.ss-facebook-color{background-color:#1877f2}.ss-twitter-color{background-color:#000}.ss-linkedin-color{background-color:#2867b2}.ss-pinterest-color{background-color:#bd081c}.ss-copy-color{background-color:#323b43}.ss-print-color{background-color:#323b43}.ss-mix-color{background-color:#ff8226}.ss-clearfix:after{content:"";display:table;clear:both}:root{--ss-wpadminbar-height:0px}.ss-on-media-image-wrap{display:inline-block!important;position:relative!important;line-height:0}.ss-on-media-image-wrap>img{margin:0!important}.ss-on-media-container{position:relative;margin:0!important;line-height:0}.ss-on-media-container.wp-post-image{text-align:center}.ss-on-media-wrapper{display:none}.ss-on-media-wrapper.ss-on-media-always-visible .ss-social-icons-container{opacity:1}.ss-on-media-wrapper .ss-social-icons-container{opacity:0;position:absolute;z-index:1;display:-webkit-box;display:-ms-flexbox;display:flex;padding:0!important;max-width:initial!important}.ss-on-media-wrapper .ss-social-icons-container>li{display:inline-block;float:left}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-ss-on-media-button{display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-ss-on-media-button span{position:relative}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-pinit-button{-webkit-box-orient:horizontal;-webkit-box-direction:normal;-ms-flex-direction:row;flex-direction:row;white-space:nowrap;font-family:-apple-system,BlinkMacSystemFont,"Segoe UI",Roboto,Oxygen-Sans,Ubuntu,Cantarell,Arial,"Helvetica Neue",sans-serif;font-weight:400;height:auto!important;width:auto!important;line-height:1!important;padding:10px 16px}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-pinit-button .ss-on-media-content{display:-webkit-box;display:-ms-flexbox;display:flex}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-pinit-button svg{width:auto;margin-right:6px}.ss-on-media-wrapper.ss-top-left-on-media .ss-social-icons-container{top:15px;left:15px}.ss-on-media-wrapper.ss-with-spacing.ss-top-left-on-media .ss-social-icons-container{left:12px}.ss-on-media-wrapper.ss-regular-icons .ss-social-icons-container>li>.ss-pinit-button{padding:12px 18px}.ss-popup-overlay{display:none;font-family:-apple-system,BlinkMacSystemFont,"Segoe UI",Roboto,Oxygen-Sans,Ubuntu,Cantarell,Arial,"Helvetica Neue",sans-serif;-webkit-font-smoothing:initial;content:"";position:fixed;top:0;bottom:0;left:0;right:0;z-index:99999;background:rgba(202,202,202,.75);opacity:0}.ss-popup-overlay .ss-copy-action{position:relative;display:none}.ss-popup-overlay .ss-copy-action .ss-copy-action-field{border:1px solid rgba(0,0,0,.1);border-radius:2px;color:#323b43;font-family:inherit;font-size:16px;-webkit-box-sizing:border-box;box-sizing:border-box;width:100%;height:48px!important;line-height:48px!important;padding:0 120px 0 50px!important;text-shadow:none;-webkit-box-shadow:none;box-shadow:none;outline:0;background-color:#fff;margin:0}.ss-popup-overlay .ss-copy-action .ss-button{font-weight:400;font-family:inherit;position:absolute;right:6px;top:5px;background-color:#557ceb;color:#fff;padding:0;line-height:38px;height:38px;width:105px;font-size:16px;z-index:9;border-radius:2px}.ss-popup-overlay .ss-copy-action svg{position:absolute;height:24px;width:24px;top:50%;left:15px;margin-top:-12px;fill:#dadada}.ss-popup-overlay .ss-popup{opacity:0;border-radius:2px;max-width:90%;max-height:90%;width:720px;height:auto}.ss-popup-overlay .ss-popup .ss-popup-heading{display:block;display:-webkit-box;display:-ms-flexbox;display:flex;-webkit-box-align:center;-ms-flex-align:center;align-items:center;font-size:16px;font-weight:300;color:#323b43;background-color:#fff;line-height:58px;height:58px;padding:0 0 0 24px;margin:0;border-top-left-radius:4px;border-top-right-radius:4px}.ss-popup-overlay .ss-popup .ss-close-modal{margin-left:auto;padding:0 24px;height:100%;display:-webkit-box;display:-ms-flexbox;display:flex;-webkit-box-align:center;-ms-flex-align:center;align-items:center;color:#a9a9a9}.ss-popup-overlay .ss-popup .ss-close-modal svg{width:16px;height:16px}.ss-popup-overlay .ss-popup .ss-popup-content{position:relative;top:3px;left:0;right:0;bottom:0;max-height:70vh;background-color:#fff;overflow-y:scroll;padding:24px 24px 20px;border-bottom-left-radius:4px;border-bottom-right-radius:4px}.ss-popup-overlay .ss-popup .ss-popup-networks{display:-webkit-box;display:-ms-flexbox;display:flex;-webkit-box-orient:horizontal;-webkit-box-direction:normal;-ms-flex-direction:row;flex-direction:row;-ms-flex-wrap:wrap;flex-wrap:wrap;margin:0 -6px}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network{-webkit-box-sizing:border-box;box-sizing:border-box;width:33.33%;padding:0 6px 6px;-ms-flex-negative:0;flex-shrink:0;float:left;display:inline-block}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a{position:relative;-webkit-box-sizing:border-box;box-sizing:border-box;height:40px;padding:0 15px 0 0;display:-webkit-box;display:-ms-flexbox;display:flex;line-height:40px;-webkit-box-align:center;-ms-flex-align:center;align-items:center;-webkit-box-orient:horizontal;-webkit-box-direction:normal;-ms-flex-direction:row;flex-direction:row;color:#fff;border-radius:2px;font-weight:500;font-size:13px;vertical-align:middle;-webkit-backface-visibility:hidden;white-space:nowrap;text-overflow:ellipsis}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a span:first-child{position:relative;z-index:2;height:40px;width:40px;text-align:center;line-height:40px;font-size:16px;margin-right:10px;background-color:rgba(0,0,0,.15);display:-webkit-box;display:-ms-flexbox;display:flex;-webkit-box-align:center;-ms-flex-align:center;align-items:center}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a svg{fill:#fff;height:16px;width:inherit;z-index:1}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a span:first-child:before{content:"";position:absolute;left:0;top:0;background-color:rgba(0,0,0,.1)}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a span{position:relative;z-index:3}.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network>a:before{position:absolute;content:"";background-color:rgba(255,255,255,0);top:0;left:0;bottom:0;right:0;z-index:1}.ss-share-network-tooltip{font-family:-apple-system,BlinkMacSystemFont,"Segoe UI",Roboto,Oxygen-Sans,Ubuntu,Cantarell,Arial,"Helvetica Neue",sans-serif;-webkit-font-smoothing:initial;position:absolute;line-height:1;left:100%;margin-left:12px;background-color:rgba(56,70,84,.85);top:50%;width:auto;white-space:nowrap;padding:8px 10px;border-radius:2px;font-size:12px;color:rgba(255,255,255,.9);z-index:2;opacity:0;-webkit-transform:translateX(-7px) translateY(-50%);-ms-transform:translateX(-7px) translateY(-50%);transform:translateX(-7px) translateY(-50%)}.ss-copy-action .ss-share-network-tooltip{left:auto;margin-left:0;right:100%;margin-right:12px;-webkit-transform:translateX(7px) translateY(-50%);-ms-transform:translateX(7px) translateY(-50%);transform:translateX(7px) translateY(-50%)}.ss-social-icons-container>li .ss-ss-on-media-button:after{content:"";background-color:rgba(0,0,0,0);position:absolute;top:0;left:0;right:0;bottom:0;z-index:1}.ss-on-media-wrapper.ss-circle-icons .ss-social-icons-container>li>.ss-ss-on-media-button{border-radius:52px}.ss-on-media-wrapper.ss-with-spacing .ss-social-icons-container>li{padding:0 3px!important}.ss-on-media-wrapper{font-family:-apple-system,BlinkMacSystemFont,"Segoe UI",Roboto,Oxygen-Sans,Ubuntu,Cantarell,Arial,"Helvetica Neue",sans-serif}.ss-on-media-wrapper .ss-social-icons-container{margin:0!important;padding:0;list-style:none}.ss-on-media-wrapper .ss-social-icons-container>li{position:relative;list-style:none;margin:0;padding:0}.ss-on-media-wrapper .ss-social-icons-container>li:before{display:none}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-ss-on-media-button{position:relative;-webkit-box-sizing:border-box;box-sizing:border-box;-webkit-box-align:center;-ms-flex-align:center;align-items:center;-webkit-box-pack:center;-ms-flex-pack:center;justify-content:center;text-align:center;width:52px;height:52px;line-height:52px;color:#fff;overflow:hidden;vertical-align:middle;font-size:16px;text-decoration:none!important}.ss-on-media-wrapper .ss-social-icons-container>li>.ss-ss-on-media-button svg{height:16px;width:inherit;z-index:1}@media screen and (max-width:782px){.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network{width:50%}}@media screen and (max-width:500px){.ss-popup-overlay .ss-popup .ss-popup-networks .ss-popup-network{width:100%}}
Rapid liquid biopsy method to capture extracellular vesicles offers avenue to affordable cancer diagnosis by StuffsEarth - StuffsEarth
Skip to content
Posted by
Alienx
October 28, 2024
Catch some EVs: A team of researchers at the University of Rochester has developed ultrathin membranes with pores sized perfectly to catch and display extracellular vesicles (EVs), tiny packets of cellular material that can provide important information about the status of the body. Credit: University of Rochester illustration
There are billions of tiny packets of cellular material called extracellular vesicles (EVs) that are produced by cells and released into each person’s blood, saliva, and other bodily fluids. EVs contain invaluable information, such as proteins and genetic material, from their original cell, which can provide insight about the status of the body. Scientists have been trying to leverage EVs for their diagnostic and therapeutic potential but have struggled to do so in a fast and cost-effective way.
In a study published in Small , researchers at the University of Rochester outline a new method for using ultrathin membranes to easily identify EVs for rapid liquid biopsies. The method, called catch and display for liquid biopsy (CAD-LB), holds promise for diagnosing cancer quickly and affordably, and assessing the progress of therapies used to treat diseases.
“By searching samples of blood or other bodily fluids for these extracellular vesicles and the biomarkers they carry, you can find important clues that something is amiss in the body,” says James McGrath, the William R. Kenan Jr. Professor of Biomedical Engineering and leader of the study.
“The idea has been around for a while, but previously it required many purification steps to isolate the EVs away from other components of the biofluid. CAD-LB is much simpler and faster, which gives it the potential for clinical use that more complex methods lack.”
The team developed ultrathin membranes with pores sized perfectly to catch EVs. Once a sample of blood is taken, it is quickly processed, injected with a pipette onto a membrane, and directly analyzed under a microscope. By counting the number of pores that glow with the biomarker for the disease being assessed, users can get a quick estimation of how prevalent the disease is within the body.
In addition to outlining the CAD-LB method, the study demonstrated the method’s ability to identify critical immune modulatory proteins on EVs. These proteins play an important role in helping the body fight tumors and can predict how well a patient might respond to immunotherapies.
“CAD-LB is currently sensitive enough to detect some cancers at a curable stage of their development, suggesting the technology’s potential for cancer screening,” says co-author Jonathan Flax, a research assistant professor at the University of Rochester Medical Center’s Department of Urology. “It may also be utilized to predict the patient-specific selection of immunotherapies, the treatment that directs the immune system to target and eliminate cancer cells.”
McGrath credited first author and biomedical engineering Ph.D. student Samuel Walker for his leadership on the study.
More information:
Samuel N. Walker et al, Rapid Assessment of Biomarkers on Single Extracellular Vesicles Using “Catch and Display” on Ultrathin Nanoporous Silicon Nitride Membranes, Small (2024). DOI: 10.1002/smll.202405505
Journal information:
Small
Provided by
University of Rochester
Citation :
Rapid liquid biopsy method to capture extracellular vesicles offers avenue to affordable cancer diagnosis (2024, October 28)
retrieved 28 October 2024
from
This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
part may be reproduced without the written permission. The content is provided for information purposes only.
Reference :
Reference link
Scroll to Top